{"id":145,"date":"2017-09-04T05:06:56","date_gmt":"2017-09-04T05:06:56","guid":{"rendered":"http:\/\/americanboard.org\/Subjects\/general-science\/?page_id=145"},"modified":"2017-09-22T21:05:08","modified_gmt":"2017-09-22T21:05:08","slug":"eukaryotes","status":"publish","type":"page","link":"https:\/\/americanboard.org\/Subjects\/general-science\/eukaryotes\/","title":{"rendered":"Eukaryotes"},"content":{"rendered":"<div class=\"twelve columns\" style=\"margin-top: 10%;\">\n<div class=\"advance\"><a class=\"button button-primary\" href=\"http:\/\/americanboard.org\/Subjects\/general-science\/specialized-cells\">\u2b05 Previous Lesson<\/a>\u00a0<a class=\"button\" href=\"http:\/\/americanboard.org\/Subjects\/general-science\/biochemistry-cell-biology\">Workshop Index<\/a>\u00a0<a class=\"button button-primary\" href=\"http:\/\/americanboard.org\/Subjects\/general-science\/viruses\">Next Lesson \u27a1<\/a><\/div>\n<p><!-- CONTENT BEGINS HERE --><\/p>\n<h1 id=\"title\">Eukaryotes<\/h1>\n<h4>Objective<\/h4>\n<p>Now we will focus on some of the diversity of living eukaryotic organisms in order to understand how life has evolved. We will start small and then move on to bigger and more familiar organisms later.<\/p>\n<h4>Previously Covered<\/h4>\n<p>We reviewed the basics of cell form and function, including the differences and similarities among prokaryotes, eukaryotes, plants and animals.<\/p>\n<section>\n<h3>Enter the Algae<\/h3>\n<p><strong>Algae<\/strong> (singular <em>alga<\/em>) are unicellular or simple multicellular organisms that photosynthesize. It is likely that the six taxonomic divisions of algae known today arose from a common ancestral eukaryotic cell. Three of the six divisions are made up mostly of unicellular organisms; the other three are mainly multicellular, and the vast majority of algae are photosynthetic. Algae range in complexity from a single cell to a filament of cells to a solid body of cells that somewhat resembles a plant. The divisions of algae are based on the presence, number, and position of flagella; their pigmentation; and other factors, like how they store food. The components of the algal cell wall, which often feels slimy due to its polysaccharides, also distinguish different algae.<\/p>\n<p>Most single-celled algae occur on the surface of the ocean or freshwater bodies, where they are known as plankton. Unicellular algae are free-living autotrophs, though some may live in colonies. Multicellular algae occur primarily in shallow waters along shorelines. Divisions of algae have been named for the algae\u2019s dominant pigment, which may hide the greening of the chloroplasts. Each Division is discussed and described below.<\/p>\n<h3>Staying Single<\/h3>\n<p><strong>Division Euglenophyta<\/strong> consists of about 1,000 species of the ubiquitous <em>Euglena<\/em>. These unicellular freshwater algae contain chloroplasts encased in a three-layer membrane, suggesting that <em>Euglenas<\/em> evolved by ingesting and incorporating green algae, which have a two-layer chloroplast membrane.<\/p>\n<p><em>Euglenas<\/em> are generally elongated, two-flagellum cells, with many chloroplasts that give the cell its striking emerald-green color. <em>Euglenas<\/em> have a photoreceptor and are remarkable in that they be either heterotrophs and\/or autotrophs. In sunlight, <em>Euglenas<\/em> are green and photosynthetic autotrophs, but put them in a dark closet and they lose their pigmentation and become heterotrophs.<\/p>\n<p><center><img decoding=\"async\" src=\"http:\/\/americanboard.org\/Subjects\/Images\/gensci\/img\/euglena.jpg\" alt=\"Generalized structure of Euglena\" \/><\/center><\/p>\n<p class=\"figcaption\">Generalized structure of Euglena<\/p>\n<p><strong>Division Chrysophyta<\/strong> includes the diatoms (approximately 10,000 species), the <strong>golden-brown algae<\/strong> (approximately 1,500 species), and the smaller group (approximately 600 species) of <strong>yellow-green algae<\/strong>. Chrysophyta are characterized by the presence of <strong>chlorophylls a<\/strong> and <strong>c<\/strong>; fucoxanthin, a yellow-brown pigment present in all except the yellow-greens; silicon-impregnated cell walls (lacking cellulose); and their storage of food as oil rather than starch.<\/p>\n<p><strong>Diatoms<\/strong> are famous for their beautiful, lacey double shells made of silica. The lacey look of the shells are actually pores that allow passage of materials into and out of the alga. Diatoms, like most plankton, are an important part of the marine food chain. Unlike their Divisional relatives, which reproduce via binary fission, diatoms sometimes reproduce sexually via a fusion of gametes, called <strong>syngamy<\/strong>.<\/p>\n<p><strong>Division Pyrrophyta<\/strong>, or fire algae, is made up of about 1,000 species of single-celled marine <strong>dinoflagellates<\/strong>. Most species are photoautotrophs, but some are heterotrophs; most have two flagella. Dinoflagellates come in a variety of bewilderingly weird forms, kept in shape by rigid cellulose walls. A few are naked, and most of these are parasitic. As their name suggests, most fire algae are red. They are often the culprits in occurrences of toxic red tides. Some dinoflagellates (especially <em>Gessnerium catenellum<\/em>) that form red tides release neurotoxins, which have caused massive fish kills and occasional poisoning of humans who ingest it in filter-feeding mussels and other shellfish.<\/p>\n<p><center><img decoding=\"async\" src=\"http:\/\/americanboard.org\/Subjects\/Images\/gensci\/img\/diatom.jpg\" alt=\"Diatoms\" \/><\/center><\/p>\n<p class=\"figcaption\">Diatoms<\/p>\n<p><center><img decoding=\"async\" src=\"http:\/\/americanboard.org\/Subjects\/Images\/gensci\/img\/dinoflagellate.jpg\" alt=\"Example of a typical dinoflagellate\" \/><\/center><\/p>\n<p class=\"figcaption\">Example of a typical dinoflagellate<\/p>\n<h3>Getting Together<\/h3>\n<p><strong>Division Chlorophyta<\/strong> contains the <strong>green algae<\/strong>, which may be either unicellular or multicellular. Chlorophyta are characterized by their <strong>chlorophylls a<\/strong> and <strong>b<\/strong> (as in plants), the presence of beta-carotene photosynthetic pigments, and their storage of food as starch. Most have cellulose in their cell walls. They are believed to be the ancestors of plants. Most green algae are freshwater organisms, although some are marine organisms; some of the 7,000 species in this Division live on tree trunks or in symbiosis with other organisms. The Chlorophyta are unique in that some species in this Division are multicellular. Scientists speculate that multicellularity may have arisen from this Division.<\/p>\n<p>Scientists believe that there are three pathways that lead from unicellularity to multicellularity. The first arose from colonies of single-celled organisms. A colony is a group of independently functioning, single-celled organisms connected to each other via strands of cytoplasm. There are four genera of multicellular Chlorophytes. In order of complexity they are: <em>Chlamydomonas<\/em>, <em>Gonium<\/em>, <em>Pandorina<\/em>, and <em>Volvox<\/em>. Volvox is a hollow sphere whose single outer layer is comprised of as many as 60,000 minute flagellated cells.<\/p>\n<p><center><img decoding=\"async\" src=\"http:\/\/americanboard.org\/Subjects\/Images\/gensci\/img\/volvox.jpg\" alt=\"Volvox are the prototypical colonial alga\" \/><\/center><\/p>\n<p class=\"figcaption\">Volvox are the prototypical colonial alga<\/p>\n<p>The second path toward multicellularity is really an intermediate form of organization, referred to as <strong>coenocytic<\/strong>. In this case, a single organism consists of many nuclei in a common cytoplasm. Nuclear divisions are not accompanied by separation of the cytoplasm or the formation of cell walls. Coenocytic algae include the large <em>Valonia<\/em> and the filamentous <em>Cladophora<\/em>.<\/p>\n<p>The third road to multicellularity is represented by algae that undergo nuclear division as well as division of the cytoplasm and the formation of cell walls. Because of this type of cell division, these algae may occur in sheets, filaments, or even a three-dimensional form. One green algae species that evinces this type of multicellularity is <em>Spirogyra<\/em>.<\/p>\n<p><center><img decoding=\"async\" src=\"http:\/\/americanboard.org\/Subjects\/Images\/gensci\/img\/spirogyra.jpg\" alt=\"Example of a mat of Spirogyra\" \/><\/center><\/p>\n<p class=\"figcaption\">Example of a mat of Spirogyra<\/p>\n<h3>Living Together<\/h3>\n<p>There are two Divisions that contain only multicellular algae, phaeophyta and rhodophyta. <strong>Division Phaeophyta <\/strong>consists of <strong>brown algae<\/strong>\u2014the seaweeds that thrive in cold marine waters. <strong>Kelps<\/strong> are members of this Division and occur, sometimes in huge water-forests, offshore in cooler climes. Many species occur along rocky shorelines, where their <strong>holdfasts<\/strong>, the kelp equivalent of roots, keep them anchored to the rocks and prevent them from being washed away by battering waves. The body of a multicellular alga is called a <strong>thallus<\/strong>, which consists of the holdfast, the stem-like <strong>stipe<\/strong>, and the leaf-like <strong>blade<\/strong>. Though these structures are likened to similar structures in plants, their internal organization is very different, and they resemble plant structures only superficially. The rockweed, <em>Fucus<\/em>, is a typical species in this Division.<\/p>\n<p>The <strong>Division Rhodophyta<\/strong>, the red algae, consists of about 4,000 species of mostly tropical and subtropical marine seaweeds (very few species are freshwater). Their red color is conferred by the presence of phycobilins, and they also contain <strong>chlorophylls<\/strong> <strong>a<\/strong> and <strong>d<\/strong>, as well carotenoids. Because of these pigments, Rhodophyta can survive at great depths, up to 175 meters below the surface. Some red algae have a slimy, mucilaginous outer layer, though others\u2014the coralline algae\u2014deposit calcium carbonate in their cell walls and are important in reef building.<\/p>\n<p><center><img decoding=\"async\" src=\"http:\/\/americanboard.org\/Subjects\/Images\/gensci\/img\/kelpforest.jpg\" alt=\"Example of kelp forest\" \/><\/center><\/p>\n<p class=\"figcaption\">Example of kelp forest<\/p>\n<section class=\"question\">\n<h4>Question<\/h4>\n<p>Eukaryotes are characterized by the presence of<\/p>\n<ol>\n<li>a plasma membrane.<\/li>\n<li>cilia and flagella.<\/li>\n<li>chromosomes.<\/li>\n<li>DNA.<\/li>\n<\/ol>\n<p><a class=\"button button-primary q-answer\"> Reveal Answer <\/a><\/p>\n<p class=\"q-reveal\">The correct answer is C. Prokaryotes have naked strands of DNA, while eukaryotes have their DNA on chromosomes in the nucleus. Both prokaryotes and eukaryotes have plasma membranes. Eukaryotes are not characterized by cilia or flagella, and all cells have DNA.<\/p>\n<\/section>\n<section class=\"question\">\n<h4>Question<\/h4>\n<p>Which of the following is a form of multicellular algae?<\/p>\n<ol>\n<li>Diatoms<\/li>\n<li>Volvox<\/li>\n<li>Chlorophyta<\/li>\n<li>Kelp<\/li>\n<\/ol>\n<p><a class=\"button button-primary q-answer\"> Reveal Answer <\/a><\/p>\n<p class=\"q-reveal\">The correct answer is D. Kelp are members of Division Phaeophyta, which contains only multicellular algae. Diatoms are unicellular algae with rigid silicon shells, so A is incorrect. Volvox are colonies of independent unicellular algae, so B is not correct. Chlorophyta is a Division of algae that includes both unicellular and multicellular algae, so C is not correct.<\/p>\n<\/section>\n<h3>Micro-Critters<\/h3>\n<p>The word <strong>protozoa<\/strong> means &#8220;first animals&#8221; and it refers to the animal-like organisms in various phyla of the <strong>Kingdom Protista<\/strong>. Protozoa are unicellular heterotrophs, mainly free-living or parasitic. They reproduce asexually via binary fission, though some have sexual cycles and others undergo conjugation, or nucleus exchange between cells. Protozoa are organized into phyla based primarily on their mode of locomotion:<\/p>\n<ul>\n<li>by flagella<\/li>\n<li>by pseudopodia (false feet)<\/li>\n<li>by cilia<\/li>\n<\/ul>\n<p><strong>Phylum Mastigophora<\/strong> contains the most primitive protozoa, which generally have two whip-like flagella, though larger members of this phylum may have many. Most Mastigophora reproduce asexually via binary fission, though some reproduce sexually or through <strong>syngamy<\/strong>. Most Mastigophora are parasites, though some are free-living; many have no cell wall and are able to form pseudopodia (brief extensions of the cell body) used for locomotion or to engulf prey. Among the parasitic Mastigophora is the flagellate <em>Trypanosoma gambiense<\/em>, which causes African sleeping sickness, transferred to humans by the nefarious tse-tse fly. Members of the genus <em>Trichonympha<\/em> are symbionts in the gut of termites and help them digest wood.<\/p>\n<p><strong>Phylum Sarcodina<\/strong> contains the ever-popular amoebas, for whom the formation and use of pseudopodia is a way of life. Locomotion solely with pseudopodia is called streaming. Amoebas create pincer-shaped pseudopodia to catch and engulf prey, a process called <strong>phagocytosis<\/strong>. The prey is then drawn into the cell and digested. The cell is sensitive to the chemical stimulus of nearby prey and streams toward its victim when it detects the prey&#8217;s chemical signals. Although amoebas are nonphotosynthetic, they do have photoreceptors. When a pseudopodium is exposed to bright light, the amoeba withdraws it and streams away. Interestingly, if an entire amoeba is exposed to constant strong light, it will eventually learn to live with it and carry on its normal behavior.<\/p>\n<p>Sarcodines reproduce both asexually via mitosis (but without breakdown of the nuclear envelope), or sexually via meiosis and the formation of gametes and zygotes. Sarcodines are usually free-living and found mainly in salt water and fresh water, and some, like the one that causes amoebic dysentery, are parasites. Some Sarcodines stiffen their pseudopodia with protective spines made from microtubules (e.g., <em>Actinosphaerium<\/em>).<\/p>\n<p>Foraminifera, the most common of all protozoans, produce beautiful and delicate shells of calcium carbonate extracted from ocean water. The shells of dead Foraminifera have accumulated on the seabed or on cliffs over time, creating million-year-old <em>foraminiferanooze<\/em>. Britain\u2019s White Cliffs of Dover are made from Foraminifera skeletons. Another Sarcodine group, the radiolarians, also has skeletons that form a radiolarian ooze on the ocean bottom.<\/p>\n<p><center><img decoding=\"async\" src=\"http:\/\/americanboard.org\/Subjects\/Images\/gensci\/img\/foram.jpg\" alt=\"Example of some foraminifera tests\" \/><\/center><\/p>\n<p class=\"figcaption\">Example of some foraminifera tests<\/p>\n<p><strong>Phylum Ciliophora<\/strong> contains another popular protozoan, the paramecium. As the phylum\u2019s name suggests, its members move by the rapid beating of the cilia on the outside of the cell. In some Ciliophora, the cilia bind together to form clumps called cirri or brush shapes called <strong>membranelles,<\/strong>\u00a0both of which actually enable the cell to \u201cjump\u201d or \u201cwalk\u201d along on these stiffened protrusions. Cilia arise from cilia basal bodies, which coordinate the even, sequential movement of cilia that propel the cell. Ciliophora have both contractile vacuoles, which rid the cell of liquid, and food vacuoles that fill with enzymes when food particles are swept into it. Ciliophora, oddly, have two types of nuclei, the micronuclei and the macronuclei, which have a very complicated mode of genetic communication. Each cell may have one or more of each type. Reproduction involves <strong>conjugation,<\/strong> in which genetic information is transferred through direct contact between the cells. During Ciliophora conjugation, micronuclei undergo meiosis, and the two cells exchange haploid micronuclei, which then fuse to form diploid micronuclei. One macronucleus dissolves, and a new one develops from one of the diploid micronuclei.<\/p>\n<p>Like amoebas, paramecia are responsive to external stimuli, including subtle changes in the temperature and chemistry of their environment. A paramecium\u2019s cilia beat constantly, directing water toward an oral groove that is forever sampling the environment. Negative stimuli, such as the presence of a toxic substance, cause a paramecium to stop dead in its tracks and beat a hasty retreat. If the negative stimulus is not so dire\u2014a change of temperature, for example\u2014the paramecium will simply turn or move away.<\/p>\n<p><center><img decoding=\"async\" src=\"http:\/\/americanboard.org\/Subjects\/Images\/gensci\/img\/paramecium.jpg\" alt=\"Example of a paramecium\" \/><\/center><\/p>\n<p class=\"figcaption\">Example of a paramecium<\/p>\n<p>The organisms in the <em>Phlyum Opalinida<\/em> are evenly covered in cilia or flagella and have at least two nuclei (though not the differentiated nuclei of the Ciliophora). Opalinids produce gametes that fuse to form a zygote. Most members of this phylum live their lives in the guts of frogs and toads, or sometimes in the digestive tracts of fish and reptiles.<\/p>\n<p>All members of the <strong>Phylum Sporozoa<\/strong> are parasites, and some, like the malaria-causing <em>Plasmodium<\/em>, are quite nasty. All Sporozoa lack cilia and flagella, and have no means of locomotion. Sporozoa produce spores as part of their complex life cycle. <em>Plasmodium<\/em> begin life in an amoeboid state, as sporozoites, in the bloodstream of a warm-blooded host. Asexual reproduction occurs in the host\u2019s liver and the resulting merozoites enter the bloodstream and cause malaria. There they reproduce asexually in red blood cells and causing the cells to explode. Eventually the merozoites enter a sexual stage, when they are called gamonts, which produce gametes. A mosquito will pick up gamonts when it bites an infected person. Then fertilization occurs and zygotes develop in the insect\u2019s gut. The oocysts (fertilized eggs) divide asexually to produce sporozoites, which travel to the mosquito\u2019s salivary glands. The next bite transfers the sporozoites to their next victim. Malaria is still one of the top five most lethal diseases in the world.<\/p>\n<p><center><img decoding=\"async\" src=\"http:\/\/americanboard.org\/Subjects\/Images\/gensci\/img\/plsmodiumvivax.jpg\" alt=\"Example of the Malaria parasite, Plasmodium vivax in a human red blood cell\" \/><\/center><\/p>\n<p class=\"figcaption\">Example of the Malaria parasite, Plasmodium vivax in a human red blood cell<\/p>\n<section class=\"question\">\n<h4>Question<\/h4>\n<p>Which phylum contains protozoa that use pseudopodia almost exclusively as their means of locomotion?<\/p>\n<ol>\n<li>Mastigophora<\/li>\n<li>Sarcodina<\/li>\n<li>Opalinida<\/li>\n<li>Ciliophora<\/li>\n<\/ol>\n<p><a class=\"button button-primary q-answer\"> Reveal Answer <\/a><\/p>\n<p class=\"q-reveal\">The correct answer is B. Sarcodines, which include the amoebas, create pseudopodia and use them to move via streaming. Most Mastigophora use mainly flagella, Opalinida use cilia or flagella for locomotion, and Ciliophora use cilia exclusively.<\/p>\n<\/section>\n<section class=\"question\">\n<h4>Question<\/h4>\n<p>The skeletons of which protozoan genus accumulate on the ocean floor to form an ooze?<\/p>\n<ol>\n<li>Paramecia<\/li>\n<li>Trypanosomes<\/li>\n<li>Radiolarians<\/li>\n<li>Plasmodium<\/li>\n<\/ol>\n<p><a class=\"button button-primary q-answer\"> Reveal Answer <\/a><\/p>\n<p class=\"q-reveal\">The correct answer is C. Radiolarians create calcium carbonate shells that accumulate on the ocean floor when the organisms die. Paramecia do not form rigid outer coverings, Trypanosomes are Mastigophora that have no cell walls (and that cause sleeping sickness), and Plasmodium are parasites that cause malaria.<\/p>\n<\/section>\n<section class=\"question\">\n<h4>Question<\/h4>\n<p>Which phylum includes the most pathogenic parasites?<\/p>\n<ol>\n<li>Sarcondina<\/li>\n<li>Opalinida<\/li>\n<li>Ciliophora<\/li>\n<li>Sporozoa<\/li>\n<\/ol>\n<p><a class=\"button button-primary q-answer\"> Reveal Answer <\/a><\/p>\n<p class=\"q-reveal\">The correct answer is D. All members of the Phylum Sporozoa are parasitic and many are pathogens. Sarcodina contains some pathogenic parasites, but most members of this phylum do not. Opalinida live symbiotically in the guts of amphibians or reptiles, and Ciliophora are ciliates that are generally neither parasitic nor pathogenic.<\/p>\n<\/section>\n<section class=\"question\">\n<h4>Question<\/h4>\n<p>Phagocytosis is accomplished by which of the following structures?<\/p>\n<ol>\n<li>Pseudopodium<\/li>\n<li>Oral groove<\/li>\n<li>Food vacuole<\/li>\n<li>Membranelle<\/li>\n<\/ol>\n<p><a class=\"button button-primary q-answer\"> Reveal Answer <\/a><\/p>\n<p class=\"q-reveal\">The correct answer is A. A pseudopodium is a temporary extension of the cell body, as in an amoeba, that engulfs prey, which is then ingested. This is the process of phagocytosis. Paramecia have an oral groove that receives food that is swept in by cilia, and also have food vacuoles that receive food from the oral groove. A membranelle is a tuft of cilia used for locomotion in Ciliophora.<\/p>\n<\/section>\n<\/section>\n<p><!-- CONTENT ENDS HERE --><\/p>\n<div class=\"advance\"><a class=\"button button-primary\" href=\"http:\/\/americanboard.org\/Subjects\/general-science\/specialized-cells\">\u2b05 Previous Lesson<\/a>\u00a0<a class=\"button\" href=\"http:\/\/americanboard.org\/Subjects\/general-science\/biochemistry-cell-biology\">Workshop Index<\/a>\u00a0<a class=\"button button-primary\" href=\"http:\/\/americanboard.org\/Subjects\/general-science\/viruses\">Next Lesson \u27a1<\/a><\/div>\n<p><a class=\"backtotop\" href=\"#title\">Back to Top<\/a><\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u2b05 Previous Lesson\u00a0Workshop Index\u00a0Next Lesson \u27a1 Eukaryotes Objective Now we will focus on some of the diversity of living eukaryotic organisms in order to understand how life has evolved. We will start small and then move on to bigger and more familiar organisms later. Previously Covered We reviewed the basics of cell form and function, [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-145","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/americanboard.org\/Subjects\/general-science\/wp-json\/wp\/v2\/pages\/145","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/americanboard.org\/Subjects\/general-science\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/americanboard.org\/Subjects\/general-science\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/americanboard.org\/Subjects\/general-science\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/americanboard.org\/Subjects\/general-science\/wp-json\/wp\/v2\/comments?post=145"}],"version-history":[{"count":6,"href":"https:\/\/americanboard.org\/Subjects\/general-science\/wp-json\/wp\/v2\/pages\/145\/revisions"}],"predecessor-version":[{"id":665,"href":"https:\/\/americanboard.org\/Subjects\/general-science\/wp-json\/wp\/v2\/pages\/145\/revisions\/665"}],"wp:attachment":[{"href":"https:\/\/americanboard.org\/Subjects\/general-science\/wp-json\/wp\/v2\/media?parent=145"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}